Fw: Sterilisation of rotary NiTi instruments within endodontic sponges. [feedly]

20. August 2015
Servus Lili,
auch kleine Erkenntnisse helfen weiter 😉

Kai Müller




Sterilisation of rotary NiTi instruments within endodontic sponges.
// pubmed: (disinfection OR sterilization OR "infection control") AND (dental OR "root canal") AND (instruments OR equipment)

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Sterilisation of rotary NiTi instruments within endodontic sponges.

Int Endod J. 2015 Aug 17;

Authors: Chan H, Tan KH, Dashper SG, Reynolds EC, Parashos P

AIM: To determine whether the following can be sterilised by autoclaving – endodontic sponges, rotary nickel-titanium (NiTi) instruments within endodontic sponges, and rotary NiTi instruments with rubber stoppers.
METHODOLOGY: Sixty four samples of eight different endodontic sponges (n=512) were placed into Brain Heart Infusion broth (BHI) for 72 hours. An aliquot of this was then spread onto Horse Blood Agar and cultured aerobically and anaerobically to test sterility at purchase. Bacterial suspensions of Enterococcus faecalis, Porphyromonas gingivalis and Geobacillus stearothermophilus in BHI were used to contaminate sterile sponges and rotary NiTi instruments (with and without rubber stoppers) inserted into sponges. The various samples were autoclaved and then cultured aerobically and anaerobically. Success of sterilisation was measured qualitatively as no-growth. The experiment was repeated with clinically used rotary NiTi instruments (n=512). All experiments were conducted in quadruplicate.
RESULTS: No sponges on purchase showed microbial growth when anaerobically cultured but some did when aerobically cultured. All autoclaved sponges and instruments (within or without sponges, and with or without rubber stoppers) showed no microbial growth. All non-autoclaved positive control samples showed microbial growth.
CONCLUSIONS: Autoclaving is effective in the sterilisation of sponges and endodontic instruments. Endodontic sponges should be autoclaved before clinical use. For clinical efficiency and cost-effectiveness rotary NiTi instruments can be sterilised in endodontic sponges without removal of rubber stoppers. This article is protected by copyright. All rights reserved.

PMID: 26280766 [PubMed – as supplied by publisher]